Member list
Current members
- Lan-Wei Yeh
- Ecology and evolution of Taiwanese Carabus ground beetles.
- Chung-Hsin Huang
- Master topic: Flucuating Asymmetry and Developmental Asymmetry of Cyclommatus mniszechi; PhD topic: Evolution of Mandible Morphology and Fighting Behavior in Stag Beetles
- Ying-Yuan Lo
- Systematics, Behavior and Evolution of Lynx Spiders.
- Leocris Batucan Jr.
- New Species and DNA Barcoding of the Mayfly (Ephemeroptera) in Mt. Malindang, Mindanao, Philippines.
- Chun-Yu Lin
- Parasitoid Wasps of Damselflies; Phylogenetics and Evolution Histories of Treehoppers
- Hsu-Yi Lin
- Fighting Behavior of Odontolabis siva parryi Stag Beetles
- Sheng Lin
- Transcriptome Profiling Psolodesmus mandarinus mandarinus Damselfly
- Pei-Hsuan Chang
- Thermal Adaptation and Habitat Preference of Psolodesmus mandarinus Damselfly
- Min-Chen Lin
- Disruptive Camauflage of Colors of Pachyrhynchus Weevils.
- Masahiko Tanahashi
- Ecology and Evolution of Pachyrhynchus Weevils.
- Su-Ping Lin
- Jun-Ying Wang
- Conservation of Mortonagrion hirosei Damselflies
- Chin-Han Hsiao
- Fighting Behavior of Rhaetulus crenatus Stag Beetles
- Wei-Zhe Tseng
- Systematics and Evolution of Weevils
- Cheng-Tse Yung
- Maternal Gift of Pachyrhynchus sarcitis Weevils
Past members
- Jo-Fan Wang
- Territorial behavior, trait evolution.
- Yu-Hsun Hsu
- Mating strategy, behavioural ecology, molecular ecology, population genetics, molecular phylogenetics, biostatistics.
- Yi-Shou Yang
- Reproductive Isolation of Psolodesmus mandarinus subspecies.
- Wataru Kojima
- Evolution of male sexual traits in Taiwanese scarab beetles.
- Zhen-Yi Chen
- Fighting behavior and reproductive strategy of stag beetles.
- Teng-Yu Liu
- Fighting behaviour and life history observation of a stag beetle Rhaetulus crenatus crenatus.
- Princess Angelie S. Casas
- Insect Diversity and Evolution
- Lu-Yi Wang
- Defense Mechanism of Pachyrhynchus weevils.
- Hui-Yun Tseng
- Aposematic coloration, island biogeography.
- Yu-Jen Yu
- Variation and Adaptation of wings.
- Yen-Ting Chen
- Systematics and evolution of insects.
- Yun-Chieh Cheng
- Damselfly larval diet, molecular evolution.
- Tai-Chia Chiang
- The effect of aposematic colors of Pachyrhynchus weevils on avian predators.
- Yong-Chao Su
- Behavioral ecology, sociobiology, population genetics, and molecular phylogenetics.
- I-Ting Hsiao
- Variation of genitalia in Euphaea amphicyana.
- Jyun-Huei Huang
- The fighting behavior of a stag beetle, Rhaetulus crenatus.
- Shi-Ting Wu
- Membracis Phyllotropis.
- Chiao-Wei Lin
- The fighting behavior of a stag beetle, Rhaetulus crenatus.
- Wei-Liang Xiao
- Variation of wing veins in Euphaea amphicyana.
- Hung-Nien Chen
- 1. The effect of typhoon on survivalship of Matrona cyanoptera. 2. Modes of phenotypic variation in Euphaea amphicyana.
- Chu-Yen Cheng
- Phylogeography of a Philippine's treehopper, Leptocentrus reponens.
- Che-Yu Kuan
- Variation of mandibles in stag beetles.
- Yat-Hung Lee
- Speciation of Euphaea damselflies.
- Ming-Yu Chen
- Phylogeography and population history of the treehoppers, Centrochares horifficus from the Philippines Archipelago.
- Shao-Chang Huang
- Visual Communication of Matrona cyanoptera
- Jen-Pan Huang
- Population Genetics and Phylogeographic Analyses of Formosan Damselfly, Euphaea Formosa (Insecta: Odonata: Euphaeidae) from Taiwan
- Wei-Yun Chen
- Molecular and Phylogenetic Characterization of Endosymbiotic Bacteria of the Froghopper, Okiscarta uchidae (Insecta: Hemiptera: Cercopidae)
Visiting scholars and students
- Jillian del Sol
- Behavioral Ecology; Mating System Ecology; Weapon Ecology and Evolution; Evolutionary Biology
- Ian Dela Cruz
- New Species and DNA Barcoding of the Stonefly (Plecoptera) in Mt. Malindang, Mindanao, Philippines.
- Shinya Komata
- Ecology & Evolution of Wing Polymorphism in a Mimetic Swallowtail Butterfly, Papilio memnon.
- Vanitha Williams
- Predatory potential of waterbug, Diplonychus rusticus and dragonfly, Diplacodes trivialis on mosquito larvae.
- Marina Vilenica
- Dragonfly composition (Insecta, Odonata) in wetland area of Turopolje region, Croatia
- Klaas-Douwe 'KD' B. Dijkstra
- History, diversity and identification of dragonflies and damselflies (Odonata).
- Erin McCullough
- " Diversification of weapon form: aerodynamic costs of beetle horns.
- Ashley E. King
- Intrasexual combat and intersexual antagonistic co-evolution in horned beetles.
Su-Ping Lin
Title
Master Student 2020-
Species: Neolucanus swinhoei
Project
Symbiotic Yeasts of Stag Beetles
Abstrct
E-mail：flyingsuping@gmail.com
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Morphology of of Neolucanus swinhoei Stag Beetles
Thermal Tolerance of Symbiotic Yeasts of Neolucanus swinhoei Stag Beetles
Abstract
Females of stag beetles (Lucanidae) possess internal mycangia to store xylose-fermenting yeasts (Scheffersomyces) and transfer them to their larvae. These symbiotic microorganisms may help the larvae to obtain nutrients from the woody materials of the habitats. However, the survival of mutualistic organisms is easily limited by the environmental adaptation of the partners. A earlier study of the symbiotic yeasts of Platycerus stag beetles in Japan shows that the different thermotolerance of the symbiotic yeast may limit the altitudinal range of the stag beetle’s habitat. Neolucanus swinhoei is widely distributed in Taiwan from lowlands to high-elevational primary forests. Because of its wide altitudinal distribution, different populations may have specific composition of symbiotic yeasts with variable thermotolerance. This study tested the hypothesis that the thermotolerance of the symbiotic yeasts affects altitudinal distribution of N. swinhoei. We expected that the thermotolerance of the symbiotic yeasts in the lowland populations is higher than that of the mountain populations. First, to investigate the species diversity and phylogenetic relationships of the symbiotic yeasts of N. swinhoei, 125 females were collected from 33 sites at altitudes between 200 and 2,000 meters. The yeasts were isolated from the mycangia and their DNAs were extracted for sequence analyses. Phylogenetic analyses based on ribosomal RNA intergenic spacer (IGS) revealed that the symbiotic yeasts of N. swinhoei can be divided into lowland (L) and mountain (M) clades, which showed a significant relationship between the altitudes of the collection sites and the clades. The L yeast clade consists of lowland populations of N. swinhoei while the M yeast clade consists of mountain populations that clusters with the yeasts of Lucanus stag beetles. Secondly, the maximum growth temperatures (MGTs) of the two clades of yeasts were examined by inoculating the identified yeast species in secondary cultures in temperature-controlled environments. The incubation experiment in the laboratory showed that the L yeast grew at temperatures up to 28°C, while the growth of the M yeast was inhibited at temperatures above 25°C. Finally, we examined the thermotolerance of the yeasts of adults in temperature-controlled environments and the yeasts collected from larval habitats. Twelve adults each were collected at 200m and 1100m above sea level, and kept in high (27°C) and low (20°C) temperatures for one week before being examined for the survival and species identification of the yeasts using diagnostic PCRs. The sawdust bootles implanted with L and M yeasts were placed in decaying woods or humus at an altitude of 200m, 600m and 1700m habitats. The bottles were recovered after 4 months to compare the survival of the two group of yeasts. Preliminary results showed that the dominant yeast species in the stag beetle changed with increasing altitudes. The difference in thermotolerance between the two groups of yeasts may affect the altitudinal distribution of the stag beetle.
Maximum Growth Temperatures of Symbiotic Yeasts of Neolucanus swinhoei Stag Beetles.
Phylogeny of Symbiotic Yeasts of Neolucanus swinhoei Stag Beetles.